Sterile chitosan folat polymer?

Hi, I want to start my cell culture to run a MTT assay , how can I sterilize my polymer?

15 December 2020 1,813 1 View

Solving chitosan folate nanoparticles in dmem?

Hi there, I have been struggling with solving my chitosan folate coated nanoseleniums in dmem culture media sincr it is only soluble in acetic acid , and hardly soluble in basic media. What can I do?

19 November 2020 5,636 3 View

TEM and SEM of chitosan coated nanoselenium?

Hi, I have a 25 ml sample of chitosan coated nanoselenium with average diametere of 112 nm, considering the fact that chitosan after being kept in water media swelles, how can I send a sample for...

08 November 2020 6,859 3 View

Addition of glycerol in measuring zeta potential?

Hi there I wanted to know if it is ok to add 2 or 3 drops of glycerol for suspending the nanoparticles in water before measuring the zeta potential?

26 October 2020 2,146 3 View

Chitosan coated nanoselenium ?

I'm running a MTT test for evaluation the effect of chitosan coated nanoselenium versus bare nanoseleniums, what are the controls for MTT assay? Should I test bare nanochitosans as well as...

27 September 2020 9,525 2 View

Why we use FBS instead of culture media in cell freezing?

Hi, I have a simple question: When we freeze cells why we use 90% FBS and 10% DMSO? I know that DMSO is used for cell cryopreservation but I don't know why we use FBS instead of culture media?

28 January 2020 7,077 3 View

How do scientists choose the best method to insert a drug into the body?

There are many methods for a drug to be used, such as oral methods (pills), anal methods, injections, etc. But when a new drug invented, how do scientists choose the best way to insert it into the...

16 October 2018 2,535 18 View

How we can remove the primer dimer, wihout alternation of desired band?

Hello everybody, I was wondering to know how we can remove the primer dimer from PCR product in a way that, the quality and sharpness of the desired band remains unchanged.

09 October 2017 9,219 9 View

Hello, if we want determine which epitopes are presented by tumor cells. what method is appliable?

18 April 2017 4,399 3 View

Can any body tell me how can i disperse Fe3o4@sio2 in water?

I synthesize Fe3o4 nanoparticles and then i modified it by Sio2, how can i make stable dispersion of this coreshell (Fe3o4@Sio2) in water? should i use any surfactant?or should i functionalized it...

14 December 2016 7,516 2 View

Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

Can bacteria and biofilm on a filter be seen with SEM without a dehydration/fixation step?

I have a filter with some bacteria and other particles that is going to be analyzed with SEM. I am wondering if anyone knows if you skip the glutaraldehyde/ethanol/critical point drying steps if...

01 September 2020 4,929 4 View

Can you help me for synthesis of morpholine usinf dehydration diethanolamine?

I started preparation morpholine dehydration diethanolamine by starting materials: diethanolamine (1mol), H2SO4 (1.8 mol). Temperature (200 oC). After 1o h the acidic solution neutralized with...

07 May 2020 6,354 1 View

Why I am getting a very large area under the curve for HMF peak on HPLC spectra, knowing that I used a suitable dilution factor?

Kindly note that we perform experiments for fructose dehydration into 5-HMF over heterogeneous catalysts. For the product analysis, we used HPLC equipped with a refractive index unit (RIU))...

22 April 2020 4,633 6 View

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10 February 2020 4,414 2 View

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01 February 2020 6,818 1 View

What concentration of Ribena is appropriate to give to mice to encourage them to drink antibiotic water?

16 January 2020 3,298 1 View

What concentration of Ribena is appropriate to give to mice to encourage them to drink antibiotic water?

31 December 2019 2,122 1 View

Can I use the molecular sieve to dehydrate the solvent while the salt composition is present?

As the title states, I would like to dehydrate the organic solvents, but we don't have the pure solvent right on hand. Just as a quick check out here, will molecular sieve adsorb or react with the...

18 November 2019 3,160 5 View

Can I mix trypticase soy broth and nutrient agar together?

I am a non-microbiologist doing some microbiology work. I have plenty of dehydrated media for both nutrient agar and trypticase soy broth. If I need to make some trypticase soy agar, without...

12 November 2019 2,893 6 View