1. There are many protocols for freezing or preserving bacteria, but the concentration of glycerol varies from 10% to 80%, many scientists use different cryo protectants. Which is the most suitable preservation method and concentration of glycerol or composition of cryo protectant?

2. Azotobacter is cyst forming and a slow grower. How many hour’s Azotobacter culture is appropriate for cryopreservation?

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