How outliers are determined in the Bioplex cytokine assay?

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Barbara Stanic

Hi,

For the values that can not be calculated from the curve(s) covering concentration range(s) of the standard(s), it is the best to do reanalysis in diluted samples (but for all of the biological samples to be compared, not only for the ones that are higher than the highest posssible calculated concentration). If you don't have more of the samples, it is fair to ascribe the ones >OOR with the highest concentration that is revealed among your samples (or if you have relatively low ones and these "extra high", you can ascribe them value from the highest concentration of the standard (if the curve looks nice, ie. if the recoveries are 90-110%). One has to bear in mind that this would be "at least that high"conentration in the samples (probably higher), so one needs to take that into account when doing statistical analysis. For the values marked with * it is fair enough to take those numbers as the concentrations quantified. Although they are out of the standard range, they are calculated according to the mathematical equation describing dose dependent curve for the standard .

Best wishes and regards,

Barbara

Lalan Kumar Arya

Dear Barbara, thanks for the answer. I was also thinking like that, but due to lack of reference I was not in a position to take a further decision. Can you please provide any reference regarding this. I don't have any more sample left, so the only possibility is to consider the value from the highest concentration of the standard.

With Regards

Arya

Dear Hager, Thanks for your suggestion and providing the reference. I think replacing the values below the detection levels prefer better to replace with the lowest detection limit rather than Zero. Because 0 itself represent some value, so it shows we did not detect any cytokine value. Am I right?

Hi Lalan,

I don't have reference for this, but I have done quite some Luminex analysis. As I said, better than taking highest standard concentration would be (in your case, becuase you show you have them) the highest calculated concentration from your samples (the highest one with *).

good luck