Would you spare 10 minutes to fill out a survey on COVID-19?

01 July 2020 8,722 13 View

Do you have time for a quick survey on psychological Factors and COVID-19?

18 June 2020 1,547 7 View

Remove antibody from coimmunoprecipitation mixture?

Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....

03 March 2019 3,494 3 View

Cracks in thermally evaporated Gold layer?

Hello everyone, I am using thermal evaporator to deposit 70-100 nm Gold (Au) layer on top of FTO substrate. The gold layer always shows lots of cracks. I have attached a photo here. I ve tried Si...

03 April 2018 2,748 5 View

How can antibodies detect SRY (sex-determining region Y) protein?

Subcellular location of SRY (sex-determining region Y) protein is in the nucleus and cytoplasm of Y-chromosome bearing sperm (http://www.uniprot.org/uniprot/Q03255). So how can antibodies act...

21 February 2017 5,850 1 View

Is it possible to model beams (using beam elements) that have non uniform section, as for instance an increasing cross section?

Hi, If yes, how can I implement it? Thanks

07 August 2014 332 4 View

How can I find the type of oxides present and their percentage based on EDX elemental analysis of a surface?

SEM/EDX Analysis

03 November 2013 5,182 8 View

Can we trust protein molecular weight markers to detect the presence of the right protein in Western blot?

In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?

17 September 2013 2,184 2 View

What do you think about using only secoundary conjugated Ab in indirect flow cytometry when you do not have the proper unconjugated isotype control?

The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...

18 December 2012 4,398 21 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View