Would you spare 10 minutes to fill out a survey on COVID-19?

01 July 2020 8,722 13 View

Remove antibody from coimmunoprecipitation mixture?

Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....

03 March 2019 3,494 3 View

Cracks in thermally evaporated Gold layer?

Hello everyone, I am using thermal evaporator to deposit 70-100 nm Gold (Au) layer on top of FTO substrate. The gold layer always shows lots of cracks. I have attached a photo here. I ve tried Si...

03 April 2018 2,748 5 View

How can antibodies detect SRY (sex-determining region Y) protein?

Subcellular location of SRY (sex-determining region Y) protein is in the nucleus and cytoplasm of Y-chromosome bearing sperm (http://www.uniprot.org/uniprot/Q03255). So how can antibodies act...

21 February 2017 5,850 1 View

Is it possible to model beams (using beam elements) that have non uniform section, as for instance an increasing cross section?

Hi, If yes, how can I implement it? Thanks

07 August 2014 332 4 View

How can I find the type of oxides present and their percentage based on EDX elemental analysis of a surface?

SEM/EDX Analysis

03 November 2013 5,182 8 View

Can we trust protein molecular weight markers to detect the presence of the right protein in Western blot?

In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?

17 September 2013 2,184 2 View

What do you think about using only secoundary conjugated Ab in indirect flow cytometry when you do not have the proper unconjugated isotype control?

The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...

18 December 2012 4,398 21 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View