Hello, I would like to apply Zombie Fixable Viability stain from Biolegend to rat full blood before FACS analyzing the T lymphocytes. I would add the Zombie stain before the additional antibodies, just as the instructions by Biolegend say. Since the lysing solution in my protocoll is to be added AFTER antibody staining, I would have to apply the Zombie live/dead stain to the full blood. Does anyone have experiences with that? I wonder what volume of Zombie stain to use per 200µL full blood.