How much volume of stock should be pipetted to get 1L medium with 5 µM hormone??

29 November 2023 1 9K Report

If I have 200 ml of stock hormone IBA (containing 1.000 μM). How much volume of stock should be pipetted to get 1L medium with 5 µM IBA?

C.A. (Kees) Kan

Your stocksolution should be diluted 1000/5= 200 times. So, 5 ml stocksolution should be added to 995 ml medium solution.

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Science method

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