Riddhi Kirit Pandya

4 Questions 4 Answers 0 Followers

Questions related from Riddhi Kirit Pandya

How much sequence coverage is needed for global DNA methylation analysis by bisulphite conversion and sequencing of LINE1 gene?

I am trying to understand the levels of methylation using LINE1 gene (surrogate marker for global methylation) in mice. My aim is to see the levels of methylation between control and test, hence i...

12 July 2019 6,971 1 View

How to separate low molecular wt protein on SDS-PAGE?

Molecular weight of my protein of interest is 7KDa. I am not able to get this on 18% SDS-PAGE electrophoresis. I want to do western blotting of my samples having this. Kindly suggest the method to...

20 March 2019 3,006 9 View

How to study the global DNA methylation?

I am working with DNA methylation currently. I am creating hypomethylation condition in the cells. so how to confirm that the hypomethylation has occurred. sequencing of only LINE-1 gene will be...

20 March 2019 3,299 1 View

Ideally, how many internal controls should be used for Real time PCR? which are the commonly used internal controls to study mice oocytes and embryos?

I am working with embryos and oocytes, i am confused about how many an which internal controls to use for qPCR? in literature, i have seen only one internal control has been used and study is...

01 March 2019 5,709 1 View