I am developing a device and I need to know how much sample in terms of volume is enough to perform a 16s rRNA analysis on gut microbiota, (i.e., microbiomes of in the gastrointestinal tract). In developed standards for performing 16s rRNA, what volume of digesta should be collected (e.g., from small intestine) to perform a successful 16s rRNA analysis on that? Is there any literature on this topic? Thanks a lot for your replies.
That might depend on the nature of samples. For details see the attached documents, please.Article Direct 16S rRNA Gene Sequencing from Clinical Specimens, wit...
Article 16S rRNA Gene Sequencing for Bacterial Identification in the...
100 ng/ul is enough but it is better if you can pool up to 500 ng/ul if you are going to sequence your samples
Dear Madison ,
Thanks for your reply. Is there any reference(e.g., paper, journal, book...) for these values 100 ng/ul or 500 ng/uL? What do you mean, "it is enough?" Can we perform 16s rRNA alaysis without sequencing?
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