I want to use it for ultracentrifugation at 55000 rpm. I have used the 1.5 ml microcentrifuge tubes at 19,000 rpm , it was fine then. I have a doubt about 55,000 rpm. Please suggest me with some info.
Hey Md Hazari,
I am no expert in these things. RCF (G-force) is always easier for me to compare. I do not know your rotor radius of the centrifuge, but 19,000 rpm should be approximatley 40,000 x g. On the Eppendorf internet page (https://online-shop.eppendorf.de/DE-de/Spitzen-Reaktionsgefaesse-und-Platten-44512/Reaktionsgefaesse-44515/Eppendorf-Tubes3810X-PF-9186.html), they say that their 1.5 ml tubes are stable up to 30,000 x g.
So I would assume that you reached the maximum with 19,000 rpm (~ 40,000 g). 55000 rpm would be approximatley (depending on the rotor radius) 338,800 x g, so way to much for a 1,5 ml tube.
Perhaps somebody with more technical experience in this field can add some information.
All the best,
Marc
I have in the past used Eppendorf vials (in that case one should pay for the brand) in a 50Ti fixed angle ultracentrifuge rotor, with plastic adaptors made in our craft shop. I found that 20,000 rpm was the maximum, at higher speeds a higher and higher percentage of vials would defragment (doi: 10.1111/j.1432-1033.1991.tb15720.x).
Perhaps, for your purpose, a table-top ultracentrifuge with rotors for small (down to 200 µL) tubes would be better, see for example https://www.thermofisher.com/order/catalog/product/46960
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