How much Resazurin powder should be added to 10 ml of culture medium to prepare a solution for a mammalian cells viability assay?

More Maxim Solopov's questions See All

Mathematical modeling of the distribution of electrical potential in the brain during the activity of neural networks, what methods would you recomme?

I am new to research, now my topic sounds like this: the problem of the distribution of electrical potential inside the brain during the activity of neural networks. There are a lot of modeling...

22 April 2024 6,112 0 View

Should data be collected exactly at the same time from different participants when using a Concurrent Multiple Baseline Design Across Participants?

Respected researchers, scholars, teachers, This doubt has been in my mind for the past couple of days. I went through several articles and book chapters but couldn't find an answer to it. While...

16 March 2024 1,191 2 View

Does anyone have stimuly for Stroop taks with numbers or arrows and can share them?

I'm interested in tasks which feature numbers and arrows as stimuli that are implemented in classical Stroop paradigm. Specifcally, number version of this task feature numbers of different sizes...

21 February 2024 160 0 View

Do You know any simple chemical kinetics for octane (gasoline) combustion?

Hello! High school work requires some simple chemical kinetics for octane or gasoline combustion (for simple 0D ICE model in COMSOL). Kinetics must contain k and E0 values or reaction rates...

15 January 2024 9,901 3 View

Does anyone know any research lab in South Korea where facility for fatty acid methyl ester analysis (FAME) of soil microbial diversity is available?

I want to analyse soil fungal diversity using FAME method

14 December 2023 5,577 0 View

Why is my profile not found on Google?

The profile under my current name is not found on Google. Only under the old name. How can I or they update it? https://www.researchgate.net/profile/Maxim-Motruk

22 April 2023 185 2 View

Is it valid to use machine translation engines, e.g. Google translate for translation/back translation procedure?

Could this be considered a valid translation/back translation procedure if you translated a questionnaire from e.g. English to Chinese using one machine tranlsation engine and then back translated...

17 April 2023 1,405 3 View

How to do electrophoresis of DNA in 2% agar correctly ?

Hello everyone. I would like to clarify and get advice on single-stranded DNA electrophoresis. A 2% solution of agarose gel was used, which contained about 12 µl of ethidium bromide. The DNA...

20 January 2022 4,861 3 View

How to automatically create topology of GPI-anchored Protein for Molecular Dynamics in Martini?

Hello! For our research we need to do simulations of GPI-anchored proteins in membrane with Martini force field. But we don't understand how to generate topology for protein with covalent...

11 September 2021 4,103 1 View

What is the name of doctor, professor B. Romeis?

In one paper I found a mention of professor B. Romeis, who wrote a book on "Mikroskopische Technik" (1936). I need to mention him in my paper, but I cannot find his full name anywhere. Even in...

13 January 2021 7,621 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View