Can anyone suggest a numerical method to calculate perimeter of a contour in charge density contour maps?

Hi i have drawn the charge density contour maps using VESTA taking CHGCAR files from VASP. Now, What I Want is that I need to know the perimeter of each contour appearing in the cross section 2D...

04 February 2021 3,637 1 View

How can I calculate flat band with valence band and conduction band potential for P-type semiconductor from negative slop Mott-Schottky plot?

I have a lot of journals regarding finding out VB and CB for n-type semiconductors from Mott-Schottky plots where MS slope is positive but I cont able to find out VB and CB for p-type...

01 December 2020 6,198 1 View

How can I calculate valence band maxima of a semiconductor using XPS?

I just need to calculate the valence band maxima for a semiconductor which is actually in a heterojunction with another semiconductor. The motivation is to find the exact energy level of VB and CB...

01 December 2020 3,774 7 View

How can I calculate the composition and quantity of different types of calcium aluminates and sulphides from EDS analysis of inclusions in steel?

How can I calculate the composition and quantity of different types of calcium aluminate (CA6, CA2, CA,...) and sulphides (CaS, MnS) from the EDS analysis result of inclusions present in Al-killed...

07 August 2020 4,621 1 View

Do I need to normalise the RNA for qPCR?

Dear all, Do I need to normalise the RNA before making the cDNA for qPCR to find out the absolute quantification in copy numbers? Why? What logic behind it when the primers amplify the...

20 February 2020 5,886 3 View

What kind of connectors and how to connect it to my small patch antenna?

I have designed a microstrip patch antenna that has a dimension of 13 mm X 8 mm. The microstrip line width is around 500 micrometers. The substrate is not PCB or FR4, for some reason, I can not...

10 February 2020 7,478 6 View

Total DNA-PK immunofluorescence on M059J cell?

Is anybody has any experience on doing Total DNA-PK immunofluorescence on M059J cells. As far I know M059J has inactivated or truncated DNA-PK protein. But can it be detectable by IF with Total...

06 February 2020 6,057 1 View

deleted the question.?

----deleted the question.

22 December 2019 4,752 2 View

What exactly we can infer from Charge Density Difference plot of a Hybrid system?

Consider a Hybrid system with two crystalline materials A and B, where is A used as a base (or substrate) and B (say a 2D semiconductor material) on top of A. For this system, a computational...

27 September 2019 4,425 2 View

Problem with the WB-F344 cel line. anyone had any experience ?

for last two months I try to culture WB-F344 cell which was purchased from JCRB cell bank. As mentioned in the documents I cultured them with MEM including 10% FBS, but after 4-6 passage the...

06 January 2019 4,021 2 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

What should be the ideal Thiolated-Oligo:GNP ratio for effective conjugation?

I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.

08 December 2020 7,975 2 View

What methods can I use to confirm if my oligo-GNP conjugate is hybridizing with my target nucleic acid?

Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...

04 December 2020 4,734 2 View

How to solve the problem with protein getting stuck in the wells during electrophoresis for EMSA?

26 November 2020 6,094 3 View

Have anyone tried to backbone digest Lenti-multi-guide with BsmBI for cloning?

Hi. I have planned to use the plasmid Lenti-multi-guide (Addgene Plasmid #85401). for mig cloning, and BsmBI as restriction enzyme...

21 October 2020 5,131 3 View

How to process fluorescently labeled oligos after HPLC purification?

Hello, I am purifyning fluorescent oligos through HPLC. My desired fraction of the mixture elutes at ~36% acetonitrile in triethylammonium acetate buffer, pH 7.0. I want these fractions to be...

23 September 2020 5,966 2 View

How to process FAM labeled oligos after HPLC purification?

Hello, I am purifying FAM labeled oligo through HPLC. The fractions I collect, are eluted in trietylammonium acetate buffer and acetonitrile mixture. I need to process these oligos, in a way...

20 September 2020 5,808 2 View

Can anyone suggest which primer(s) (oligo dt/random hexamer or both) would be good to generate E. coli cDNA?

Knowing that very low percentage of E. coli mRNA is polyadenylated, perhaps random hexamer should also be used to generate even the 1st strand of cDNA, instead of oligo (dt) primer?

29 August 2020 6,515 3 View

Can oligo hexamer primer produce sequencing error?

Hello everyone I conducted sequencing prosedure for RNA genome segment (about 700nt) , for this reason I employed the random hexamer primer for cDNA synthesis . after sequencing (by sanger...

16 August 2020 3,994 3 View

Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligo nucleotide in solid phase?

Hi, anybody help me? Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligonucleotide in solid phase? What's the consequences?

28 July 2020 7,993 3 View