What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

How can you alter line thickness in emmip plot (R)?

I am working with R and plotting emmeans graphs, yet I cannot find online how we can change the line type or its attributes using the emmip function. I have two independent categorical variables,...

27 October 2020 9,339 1 View

How to synchronize two stepper motors most optimally?

We need to push a large load (2 tones) using two stepper motors, connected rigidly to the axis (two screws). I'm worried the motors could micro-step out of sync. And cause malfunction. I can see...

21 October 2020 6,363 4 View

How to fix convergence problmes using Mohr Coulomb Plasticity?

Dear colleagues, I am trying to simulate a strip foundation on a cohesionless soil with a friction angle of 30 degrees. However, I obtain convergence problems. I am using a geostatic step. The...

21 October 2020 4,556 2 View

Can you integrate Zotero with this website?

Is it possible to integrate Zotero or any other bibliographic software application with this website application? If not, is it possible to do that with academia, ssrn or Google Scholar? Any...

21 October 2020 4,712 1 View

How to find out, if a recent Sentinel-2 acquisition failed or had a problem?

Hello, I am currently waiting for an image to appear on Scihub and it takes longer than usual (acquisition date 2020-09-22, orbit r122, T33UWR tile actually, but looking for general answer). So it...

23 September 2020 6,842 5 View

Can small law firms adopt AI?

After considerable research, I have found several use cases which can be used to exemplify some implementations of AI on law firms. However, there is clearly a disadvantage for this segment when...

01 September 2020 7,132 5 View

How can i model a shock tube with 2 different gases on Fluent?

Hi, I'm currently trying to study shock tube analysis on fluent and I have modelled a shock tube with a driver section (high-pressure) and a driven section (low pressure). What I want to study is...

22 July 2020 8,162 3 View

How can i make an isopeptide bond in GROMACS ?

Hi everyone I'm new in all the field of molecular dynamics, but i trying to run a SMD of one protein with two isopeptide bond, one in each domain, that prevent my protein stretch. My problem is...

07 July 2020 6,201 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View