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1ml of each bacteria culture were reinoculated into new sterilized conical flasks containing 50 mL of Tryptone Soya Broth. 3 mL suspended E.coli from conical flask were pipetted and transferred into the cuvette and directly analysed using uv-vis to check absorbance. Absorbance (OD600) were taken for each 1 h until reached 4 h during incubation time. Wavelength with 600 nm was selected. Then distilled water and TSB was used as blank and control respectively. The Growth curve versus incubation time was prepared. The whole process was repeated for gram positive bacteria, S.aureus. But If I want to check optical density microbe that mix with sample extraction. How much sample solution dragon fruit peel extraction I should add into the 50ml TSB that containing microbe?
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- Analytical Chemistry
- Extraction