How much affect label qPCR tubes?

07 December 2023 0 4K Report

I was doing qPCR in individual tubes and when I had everything done I realized that I should not label the tubes, so how much interference i will have in the results?

More Rayén Amaranta Aguilar's questions See All

Can someone help me with this article?

Vasquez Moreno L. L. Monteagudo Toranzo S. Vera Lima R. . 2002 . Incidencia de Xylosandrus compactus (Coleoptera: Scolytidae) en diferentes variedades de Coffea en condiciones...

10 April 2024 5,487 0 View

How to obtain the standard deviation of multiple sample scans in FTIR ATR Cary 630?

Hello! Does anyone know where can I find the standard deviation out of the multiple scans done in FTIR ATR using Cary 630? For my method, I've selected 64 sample scans, so as to improve the signal...

02 April 2024 9,690 2 View

Any protocol for loading dsRNA in an agarose gel?

I am expressing dsRNA using E.coli as vector. I would like to check that my bacteria culture is expressing dsRNA. I am planning to extract RNA from my bacteria culture and then load the total RNA...

18 March 2024 1,371 3 View

Is there a method to stain ingested bacteria?

I am working with a marine non-model organism that has a planktrotrophic larval type and I found out that the larvae are not very selective while feeding. I am planning to do RNAi by feeding them...

25 January 2024 7,678 3 View

Any thoughts on why I'm getting the same DTA+ TGA thermograms out of two different standards?

I'm working with TGA (Thermogravimetric analysis) + DTA (Differential thermal analysis) of milk proteins on a STA2500 Regulus equipment by Netzch. I have two standards: one of B-Lactoglobulin and...

18 January 2024 2,383 1 View

Riddle me this?

Here's something to keep you busy over the holidays. Merry Christmas everyone! (Maybe Santa Claus will bring you an Alvin compass to help you do the geometry problems in my book.)

23 December 2023 3,937 0 View

Can Agilent 7100 Capillary Electrophoresis instrument be plugged in directly to a power source? Or it needs UPS?

13 November 2023 3,631 4 View

Is it possible to analyze single nuclei RNA seq libraries with an assembled transcriptome or only with the genome?

I am trying to generate single nuclei RNA seq libraries from a non-model organism. We have a genome that it is not well annotated and a bit incomplete. I know that for single cell RNA seq is...

02 October 2023 5,180 1 View

I would like to know how to lyse C. elegans?

Does anyone have a protocol to lyse C. elegans and any brand of antibody against GFP that has worked for this model?

17 September 2023 2,561 2 View

Phytagel vs. agar in plant tissue culture?

Hi everyone. Is the use of Phytagel better than agar in Plant tissue culture? I work with stevia and sugarcane, does anybody have experience in these crops in vitro? Or does anybody prefer the use...

01 September 2023 8,149 6 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

Method to prevent crosstalk fluorescene signal between 2 dye labeled microparticles?

My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....

19 June 2024 2,680 1 View

Please share the HNMR sample preparation procedure for isotopic labelling test to confirm source of N2 during ENRR to ammonia.?

During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.

29 May 2024 5,165 0 View

Can FITC fluorescence stay in conjugated proteins after running a Western-Blot?

I have FITC labeled proteins, and I would like to detect its fluorescence after running proteins western blot. Does fluorescence quench in WB condition? so I can detect FITC labeling (without...

27 May 2024 4,087 3 View