How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

How to generate MATLAB code from ANFIS model?

Dear scholars, I was wondering how can I generate MATLAB code of an ANFIS (FIS) model? I need to integrate my ANFIS (FIS)/Fuzzy logic system into another MATLAB code to render optimizations. Do...

04 February 2021 6,098 4 View

Please ,How can I correct this kind of error?

27 January 2021 4,815 5 View

Compare to prevalence before and after covid in population studies?

Dear researchers, I have a prevalence of some diet habit in relatively large narrow population of children 13 years old in 2019 and 2020. Logically they are different population. However, since...

10 January 2021 3,170 9 View

Will programmable logic controllers (PLCs) be replaced by IOT devices as the latter become more reliable?

Will programmable logic controllers (PLCs) be replaced by IOT devices as the latter become more reliable?

29 December 2020 255 6 View

Explain the strain determined using directly the tensile machine test or the video extensometer ?

Dear Researchers, I would like to know the logical margin between the strain of a polymer in the tensile test determined through the traverse (the value from the machine itself) or using a video...

12 December 2020 3,636 4 View

Does multivalued logic is necessary for artificial intelligence?

Fuzzy logic is multivalued. Does the multivalued logic is necessary for artificial intelligence?

26 November 2020 5,572 6 View

Hypothesis for my dissertation - M&A?

Hi all. Trying to settle on a stellar dissertation for my MBA program in the area of finance. I am looking at M&A and how it has helped companies in the financial services sector survive...

24 November 2020 3,182 3 View

What is neutrosophic similarity score (NSS) used for segmentation in image processing? How can it be implemented in Matlab?

Neutrosophic Similarity score is similar to Fuzzy logic. Few papers report using it for image segmentation but implementation details are not provided. If any material regarding this on how it can...

05 November 2020 2,859 3 View

How can I compare two groups with hierarchical regression after finding no interaction?

16 October 2020 8,029 2 View