Are there any interesting areas of research yet to be studied with rice viruses?

I'm currently in the process of coming up with a research pitch for a lab to be funded here in the Philippines. Rice is quite the common crop in the South East and its diseases are commonplace....

23 November 2020 5,194 4 View

Why is my Tet on system constitutively active and not dox inducible ?

I am using a Tet on system to overexpress a LncRNA in mouse ES cells, based on a piggybac vector (pXlone). I performed transient and stable experiments. In both cases, the LncRNA is expressed even...

13 September 2020 8,303 1 View

Have you found an arithmetic ratio of form 3/2 in your investigations in physics, quantum physics, genetic code, chemistry and other fields?

Do you think then that there is a universality of this particular ratio? I, for my part, highlighted this ratio in these different areas: Genetic code, quantum physics and the 3/2 ratio The...

19 August 2020 3,070 5 View

Generalization of a quantitative study?

I conducted a quantitative study addressing 340 executives. The administration of the questionnaire was held by a panel company. The analyses were conducted using Smart-PLS. A reviewer asked me:...

22 July 2020 7,099 2 View

How to estimate the control value in Direct collocation MPC?

Hi dear researchers, I am currently implementing direct collocation NMPC, However, I have a doubt on the control value for the first control u_0 that should be applied to the system once the...

28 July 2019 1,783 3 View

Dear researchers, I get my UV-Vis DRS results of my materials. So i get F(R), is it allow to plot tauc question directly using F(R) withou absorbance?

i have F(R). can i plot tauc equation with F(R) without convert it into absorbance? What represent F(R)? is it also absorbance according to K-M equation?

05 May 2019 1,847 1 View

What is the suitable way and typical procedure to obtain graphitic carbon nitride from urea ?

02 January 2019 2,367 1 View

Univariate or Multivariate Normality. How to choose the normality test when using PLS-SEM or CB-SEM?

29 November 2018 8,686 16 View

Zebrafish cell line ZF4 best expression promoter/vector?

I never worked with non-mammalian cell lines, I just ordered ZF4 zebrafish cell line and I am looking for a good expression vector and I have no idea which promoter would yield the most expression...

20 September 2018 8,286 1 View

In your country, does any researcher study old handbooks of agriculture, to interprete the archaeological remains related to the rural world ?

I read old agricultural books (XVI to beginning of XXth C.) to get a better understanding of archaeological remains bound to agriculture. I wish to know if someone else is using the same approach...

01 February 2017 6,730 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View