Hello Diana,

Assume the TLC is 10,000/ microliter (range 6-14) and the Lymphocyte and monocyte percentage (65 +5) as there is relative higher lymphocytosis at this stage of infant's life. The PBMC expected may be as high as 7 million/ ml. However usually the yields are lower and the common dictum is that  from 1 ml of adult blood  one gets up to a million cells. Hence you may get only about 2 -3 million cells per ml of a two month old infant. 

Thanks so much its a very useful information !

Great Answer Prof .Mahendra Narain Mishra

thank you