Mahendra Narain Mishra

11 Questions 4 Answers 0 Followers

Questions related from Mahendra Narain Mishra

How much do you rely on Luminex Crossmatch (donor lysate assay) for pre and post transplant work up?

Luminex Crossmatch popularly known as DSA is popular in limited constraints settings. It is associated with high background, many false positive especially HLA -Class II IgG antibodies and to some...

25 February 2025 7,710 0 View

How frequently do unsensitized cases show a positive SAB HLA antibodies with negative pooled /phenotype bead assay in unsensitized cases? Approach?

Some samples from unsensitized patients as per history were negative on pooled bead assay, but positive by SAB assay using kits from Werfen. The kits don't detect DPB1 reactivity due to...

25 February 2025 5,949 0 View

Anti HLA C antibodies and shared HLA -Class II alleles in Recurrent abortions?

I want to know whether clinically any one is looking for anti HLA -C antibodies against spouse / Partner in Recurrent abortions? Which method do you find more appropriate Flow crossmatch or...

30 January 2024 8,654 1 View

Has anyone observed loss of HLA class I reactivity from patient serum if not refrigerated for a week and kept at 40 deg Celsius?

Our laboratory uses External Proficiency samples which have both HLA -class I and II reactivity. Three samples that were kept at 37-40 deg Celsius in Customs Warehouse for a week due to delay in...

24 June 2023 5,864 0 View

I want to know more about the products EasySep™ Human T Cell Isolation Kit and EasySep™ Human B Cell Isolation Kit for CDC crossmatch?

I want to know more about the products EasySep™ Human T Cell Isolation Kit and EasySep™ Human B Cell Isolation Kit. Requirement is to use it for CDC crossmatch using eosin staining and Formalin...

02 March 2023 9,372 2 View

What is the best method for determination of uncertainty (MU) for all parameters for a hematology analyser ?

Recently a six part Sysmex XN -350 was installed in our laboratory . On inquiring regarding MU for all parameters, the application specialist mentioned that whole blood was diluted with Cell...

31 October 2020 5,313 1 View

What do we use for Mixed Lymphocyte reaction - PBMC or B cells or T cells ?

Mixed lymphocyte reaction is a measure of HLA - Class II disparity . This is not expressed on T cells unless induced and the percentage of B cells is 15-20 % in PBMC.

02 June 2018 3,468 3 View

ESR by Westergreen Method with Sodium Citrate as against EDTA yields higher value. Our results were contrary to this . Request inputs please?

We were using EDTA as anticoagulant for long instead of documented Sodium Citrate for ESR by Westergreen Method . On comparison with Sodium Citrate tubes we found that the values obtained by using...

05 April 2018 3,552 1 View

What is the best method to prevent tailing of neutrophils in a Peripheral Blood Smear ?

In my lab , very often the distribution of neutrophils is not uniform and over 80% are present in the tail . I want to know how to prevent this tailing .

14 October 2017 9,356 2 View

How long are B lymphocytes viable from whole blood in Sodium Citrate vacutainer ?

It has been observed that the viability for some samples is

17 June 2016 9,112 3 View

Epidemiology of SLE in India

The incidence of SLE in developed nations is 1 in 2500 and for women in reproductive age group it 1 in 700 with higher incidence in blacks and Hispanics. As per a paper of 1993 cited below the...

01 January 1970 707 0 View