How many mice are needed for long term potentiation?

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How to correct my name?

How can I correct my name on my profile?

30 June 2024 10,166 1 View

Is the Gamma function completely monotone within the interval (0,1)?

I am wondering if the Gamma function is completely monotone within the interval (0,1). I use Mathematica to plot the derivatives of Gamma function up to 10th order of derivatives and the results...

29 May 2024 2,846 3 View

Why do endothelial progenitor cells grow better under this high glucose condition?

Dear community, I am going to study the effect of a hypoglycemic drug on endothelial progenitor cell (EPC) functions. The published work has reported that a 24h 25~33mM glucose treatment is enough...

11 April 2024 8,833 0 View

What is the longest duration for whole-mount in situ hybridization staining in mouse embryos?

I'm conducting whole-mount in situ hybridization (WISH) on mouse embryos using probes that I transcribed from PCR products. In the final staining step, I incubated the embryos at 37℃ for two days...

02 April 2024 192 0 View

Any existing technology for the conversion of butene/propylene to dodecene?

Exploring possible technologies offered by companies for the conversion of butene to dodecene by polymerization, with olefin conversion rate >90% and selectivity > 50%.

02 April 2024 6,546 0 View

Is polyglycolic acid being explored for its use as support material in 3D printing?

PGA seems to be promising with its biodegradability, is that driving its research for use as support material? Are PGA's completely soluble in water?

03 March 2024 6,917 1 View

Is carprofen a suitable painkiller for animal postsurgical pain management in this situation?

I have a mouse skin wound healing model, and it's suggested to give pain management after wounding procedure. It's known that carprofen is a nonsteroid anti-inflammatory agent, acts as a...

21 January 2024 6,808 2 View

After IP, the protein we targeted for the study was 30 KDa above original molecular weight, is this a true situation because of protein form change?

We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...

04 January 2024 6,926 0 View

Panel data Regression for unbalanced Data?

Hi Guys, I have been working with panel data for my research. One of the problem I faced is that. My dataset is monthly based from 2010 to 2023. I was able to do a yearly analysis but for monthly...

29 November 2023 5,140 0 View

Is Surangin (a coumarin) available in market as a hptlc marker? In India?

Surangin is a coumarin. Wanted to know if it is available in Indian market as a standard/marker?

25 October 2023 8,499 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Paula Parra

We normally use around 10 animals, with good slices, as final n. So I will say 15-20 per group, considering you will have bad slices days!

Sharon Ding

Thank you Paula!

Usually how many slices you use for LTP? I found someone use 8 someone use 6.

Sharon, i do LTP in two rigs in parallel, so I use 6-8 depending and I still leave at 5-6pm 😀