How many mice are needed for long term potentiation?

More Sharon Ding's questions See All

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

What is th needed sample size for a one-factor convergent validity check?

Hi all, I recently posted a question about how to conduct a convergent validity analysis for a self-developed self-esteem scale. I got some very useful responses! However, something I am stuck...

01 December 2020 4,370 6 View

Is there a method or developed technique for soil winnowing?

The light fraction (undecomposed organic matter) needs to be removed from prepared soils (

02 June 2020 921 5 View

Is there other related study about the impact on COVID-19 on Kindergarten Academic activities?

Kindergarten learners with internet connectivity at home are also encouraged to access enrichment activities. How mdifficult the new normal is, if connection is not possible, worst if there is no...

10 May 2020 10,019 3 View

Does anyone happen to know how to deal with kernel random matrix without using any expansion (i.e., Taylor, series, orthogonal or bi-orthogonal)?

The literature all depends on some kind of expansion. It is useful for global analysis but not for local.

28 January 2019 6,589 1 View

My next study will be in a child autism class course what are some of the basic questions I will see on text and test papers?

Reason I ask is because I can find people that may be interested in the subject I have submitted my self to a fund raiser for autism on the Facebook network. any people interested in donating to...

27 December 2018 5,917 1 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

Gaussian optimization terminating without error message?

Hey, I have been trying to optimize the structure of what I guess I would call a lignin derivative. The system contains 63 atoms and has no charge. I have tried using DFT and HF using a 6-31g(d)...

06 November 2018 2,781 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

Paula Parra

We normally use around 10 animals, with good slices, as final n. So I will say 15-20 per group, considering you will have bad slices days!

Sharon Ding

Thank you Paula!

Usually how many slices you use for LTP? I found someone use 8 someone use 6.

Sharon, i do LTP in two rigs in parallel, so I use 6-8 depending and I still leave at 5-6pm 😀