How to publish a diagnostic method?

I work at a diagnostic lab and have developed methods that can be used to decrease the amount of time it takes to get results for a test, is there a format for publication with this kind of project?

16 March 2020 4,561 4 View

Can I change group size of Hosmer-Lemeshow goodness of fit test in SPSS?

I am using SPSS to run a Binary Logistic regression. I have the option of choosing Hosmer-Lemeshow goodness of fit test but see that SPSS chooses the default group size of 10 for the test. I want...

15 January 2019 7,441 3 View

What are options for cell counting chambers for darkfield microscopy?

I have seen that the Petroff-Hausser chambers work but they are cost prohibitive for my lab. Are there other options?

30 August 2017 8,141 1 View

What type of questions can be asked to juveniles if we want to gather information from them about abuse they faced and their relations with family?

09 October 2015 2,439 5 View

What is a reliable statistical model for reach curve prediction of an advertisement exposure over time?

I need some guidance concerning reach curve prediction.

28 October 2013 2,811 2 View

How do we obtain free protons from the electrolysis of water while moving said protons into a free standing platinum lattice structure?

In an electrolysis system, How do I obtain hydrogen gas from water in order to move the H2 into a free standing platinum plate? For the electrolysis system, we may use palladium for the cathode,...

01 January 1970 875 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View