Fisher is out of Mueller Hinton with 5% Sheep blood, will using Chocolate Mueller Hinton be acceptable?
This medium is intended to used for cultivation of fastidious organism e.g. Haemophilus. What organisms that you're going to do antimicrobial susceptibility testing?. I don't think CLSI has an interpretation zone by using this medium.
If your intention is to cultivate fastidious organisms that require X (hemin) and V (nicotinamide adenine dinucleotide - NAD) growth factors, then you can use chocolate Mueller Hinton agar instead of Mueller Hinton with 5% Sheep blood. However, if you want to Determination of hemolytic activity of the organism then you cannot use chocolate Mueller Hinton. If you want to conduct susceptibility testing, you might need to supplement your medium with VCNT and other vitamins, depending on the type of organism you are cultivating.
Good Luck,
Chocolate Mueller Hinton Agar has been developed for fastidious organism susceptibility testing , originally for Haemophilus species and A. pleuropneumoniae and H. somni (both veterinary pathogens).
Currently the CLSI suggests the use of Haemophilus test medium for H. influenza and H. parainfluenzae species.
If you are testing streptococci I would suggest that it is not appropriate as it has X and V factors which were added to this media specifically to allow the organisms above to grow.
CLSI quality control ranges and interpretive criteria for Streptococci, Listeria and N. meningitidis were developed using 5% sheep blood Mueller Hinton agar . So I would not recommend the use of chocolate Mueller Hinton for these species.
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