06 September 2019 1 2K Report

Hello,

I am working on a project with knockdowns. Is there a universal amount of knockdown one start with? Is there anything in literature to support this? I'm having trouble finding anything.

Thank you for all your help!

More Joanne Chan's questions See All
"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

La animación digital en plataformas digitales?

Hoy la animación se utiliza como una tecnología multimedia con gran potencial educativo, que va mucho más allá de sólo crear figuras, ya que puede promover una mejor comprensión en...

01 August 2024 7,186 0 View

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

01 August 2024 8,217 1 View

How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

30 July 2024 1,607 1 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

After a lot of feature engineering for CTR modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing?

After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?

29 July 2024 4,955 0 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

All math can be explained by iterator of code?

all math can be traversed by code? all math can be translate to code?

26 July 2024 9,530 0 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Why electrical charge on the moving plate increase?

Hi, everyone This figure depicts a simulation of an electrostatic energy harvesting system in COMSOL Multiphysics software. My question is regarding the relationship between the changes in...

19 July 2024 4,694 4 View

Similar questions and discussions
Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

My question is about a barley seed ?

I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.

26 June 2024 3,451 2 View

How to avoid errors while doing qpcr?

I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...

07 June 2024 7,292 1 View

Lentiviral Transduction Issues?

Hi there, I am attempting to transduce cells with the TLCV2 plasmid containing our sgRNA of interest. My transfection of the HEK 293T cells seems to yield positive results as the EGFP is clearly...

06 June 2024 2,131 1 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?

31 May 2024 454 2 View

How to handle missing data for proteomics data?

I have a proteomics dataset with missing values. I tried some strategies, but the point is that there are sets completely with missing values. The last strategy was to apply MissForest in python...

29 May 2024 5,563 4 View

I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?

I will like to knockdown two genes using shRNA what is the best method and plasmid I can use?

21 May 2024 5,796 0 View

SiRNA services in India?

I am starting out on some siRNA based knockdowns on primary cells. Would anyone recommend good services they have used in India and any advice considering the advantage of modified and fluorophore...

09 May 2024 6,433 0 View

Are there any international companies that provide LC-MS/MS proteomics services to whom we can outsource our samples??

We want to find interacting protein partners for our protein of interest.

28 April 2024 921 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close