I'm using cd45, cd34, and collagen-1 as markers. The question is, how many fibrocytes would I expect to see in blood from a healthy individual and consequently how many events should I include in the flow cytometry?
Preethy .V
set the events based on the good population you get during analysis. usually 2 % of the population are taken as valid results. so usually 10000 events are kept to get 2% cell population . Before analysis make sure your sample had one million cells for flow cytometry analysis.
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