Hello!
I was wondering how many Human Endothelial Cells/well are usually used for MTS assay using Human Endothelial Cells!
Thank you for your time!
What is your purpose of MTS assay? for testing cytotoxicity or growth alteration? Your endothelial cell is commercial cell line or primary cell line? Do you know about their metabolic activity?
Different cell lines (even though they are all endothelial cells) and different purpose will affect your initial cell density.
If you still have no idea about it, you can use a serial numbers of initial cell density (maybe 1500, 3000, 6000, 10000, etc) and collect the MTS RAW data. That you will have some idea to choose an appropriate cell density for your experiment.
Hello Woori Lee
You will have to optimize the seeding density by running a pilot experiment with different cell densities. If you are using a 96-well plate for MTS assay you could try various seeding cell densities starting from 2000 cells per well and go up to 20000 cells per well. The seeding density will depend on the cell type and its growth rate as well as the number of days of your experiment.
Choose a cell density that will be at least 70-80% confluent on the day of treatment, so that they do not get over confluent on the final day of the result as this could affect your readings.
Best.
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