Hi,
I would like to know how many controls can I compare to my samples in GEO2R? What I mean is, if I choose 10 samples should I compare them with just 10 controls or It is possible to add more controls , if the microarray study provided. For example: 10 samples/ 20 controls
and how about visa versa?
Another thing is, for gaining significancy (adjusted p-Value) , is it possible to select controls or samples that are more similar in expression , or I should select them randomly? Could it be a kind of normalization?
Thank you very much