The purpose of this study is to obtain plasma membranes from adherent cells by means of ultracentrifuge. The problem with the experiment is that the yield is too low when obtaining plasma membranes.
I'm thinking of increasing the amount of cells to increase yield, but I'd like to ask how much the cells need. I have looked through several papers and expect 10 ^ 9 to be needed. I think using a kit will yield a high yield with a small amount of cells. I want to know how many cells are needed for isolation.
Long ago, I isolated plasma membranes from goat mammary gland in my Master's dissertation. In my opinion, you need several billions of cells to get enough material to analyze. I will suggest you optimize yourself. Start with one concentration and start increasing till you are satisfied with output.
Best regards,
Subhash C. Juneja
In the end, we need to prepare the maximum amount of cells to optimize the experiment. Thank you.
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