Hi Bostjan,

For determination of Antioxidant enzyme activity or oxidation product you need 50,000 to 500,000 Cells. For determination enzyme activity, as you know, we encounter to signal amplification and thus we need lower cell number (10,000 to 100,000, mean 50,000). But for determination of oxidation products such as MDA, you have not amplification and can determine MDA directly based on TBA reaction, so you need more cell number (100,000 to 1000,000 mean 500,000 cells).

For solid tissue you need average 100 mg tissue for preparation a homogenate 100 mg/ml.

After homogenization, centrifuge and separation supernatant, you can determine antioxidant enzyme activity or oxidation products.

best

mehdi

Thank you very much for your answers.

you're welcome.