How many CD8+ cells will be obtained from a 10 ml whole blood sample?

02 February 2015 1 4K Report

I will extract total RNA from these CD8+T cells. So I also wonder it what concentration of RNA molecules can I obtain?

If you take 10ml blood and separate it on FH density gradient you may get 2-3 million PBMCs per ml i.e.; 20-30 million cells and out of which about 60-75% will be CD3 i.e; around 12-15 millinon . So ultimately you may get around 30-40% of CD3 positive cells as CD8 which will be around 3-4 million.

These number of cells may be enough to get good RNA to do further experiments

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