How many base pairs of 16S ribosomal DNA to be sequenced are the optimum to be analyzed by NCBI?

More Johan Prabawa's questions See All

Please suggest literature reviews on public sector innovation capabilities?

I am looking for literature reviews focusing on public sector organizations capabilities for participating in and coordinating innovation together with both private and non-governmental organizations

09 June 2024 7,818 5 View

Is E = mc^2 the potential energy of a mass m at the cumulative gravitational potential originating from remote masses of the universe?

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29 December 2023 1,242 15 View

Are there any scales for body image satisfaction with interpretation, scoring, and questions that I can use?

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What scales can I use to measure an adolescent's satisfaction with its body image and to measure an adolescent's social phobia levels?

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15 October 2023 656 3 View

How do I compute range in a likert scale with 0 to 4?

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29 September 2023 7,535 3 View

How to correctly describe and model the diffuse reflectance measured from a set of optical fibers in skin?

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Fractography - how do I clean a charred fracture surface?

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How to troubleshoot failed salt test in passive conjugation of AuNP and antigen?

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Problem with printing. How can I revise one of my papers published by Research Gate?

Some of the equations in one of my papers did not print right. How can I fix them?

01 May 2023 9,447 0 View

How do I update one of my published papers?

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01 May 2023 8,347 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

Which Scopus Journal provides the most affordable fees?

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Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

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Who will be moral responsible for the death of thousands of people in the event of an earthquake?

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08 August 2024 6,134 12 View

How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Are there any instruments for studying time similar to the way it is in space?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Slimane Choubane

I think that it doesn't matter, because when you will blast it, it will be compared to the whole database anyway and you will get your matches. However, it is clear that it is not enough to identify your strain and it will be a partial identification.

Avinash Sharma

Hey, I think just for identification purpose it should be ok but if you want to work on the taxonomy then you need to have around 1400bp 16S rRNA gene sequence. I will recommend EzTaxon (http://www.ezbiocloud.net/eztaxon) for the comparison. NCBI doesn't have any quality criteria for the sequences while in EzTaxon all the sequences are related to type strains only, which makes it better in quality of the sequences.