Johan Prabawa

4 Questions 5 Answers 0 Followers

Questions related from Johan Prabawa

How many base pairs of 16S ribosomal DNA to be sequenced are the optimum to be analyzed by NCBI?

I've sequenced a 16S rDNA and the result is I only get 599 bp from approximately 1500 bp of the DNA. Is it good or valid to be analyzed by NCBI? I think the long of my 16S rDNA that readable is...

04 January 2016 1,184 2 View

Why competent cells of E.coli DH5α cannot grow after transformation?

I have made competent cell of E.coli DH5α with CaCl2 method. This is the following method: 1. Grow the cell on 50 mL Luria Bertani Broth until OD600 between 0.3-0.6 2. Aliquote the cell culture...

16 October 2015 4,386 9 View

Can PCR product be used for template in PCR reaction?

I have done PCR of 16S rRNA from bacteria genome. I used 27F and 1492R primer for the PCR. The concentration of the PCR product is small, so I want to repeat again the PCR with PCR product before...

25 June 2015 6,888 20 View

How can I isolate extreme acidophile bacterial of acidic water from crater lake?

I collected soil and water from the crater lake. Location of the sample I took at Ijen Mountain, Banyuwangi, East Java, Indonesia. The water is extremely acid that the pH is + 0-1. I want to...

28 May 2015 3,646 8 View