How long would you need to treat adherent melanoma cells with 20uM amsacrine to induce apoptosis, for use as a positive control?

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Experience on Data for Master Thesis on Disposition Effect in Social Trading Platforms?

Dear Forum, My master thesis investigates the disposition effect—where traders sell winning shares too early and hold onto losing shares too long—on social trading platforms (STPs) like eToro,...

08 July 2024 187 0 View

Assay cascades - how do they work in DMTA cyle?

Could you explain to me, how do assay cascades work during the DMTA (Design-Make-Test-Analyze) cycle of Drug Discovery? What is involed in it? How many assays are usually present?

24 June 2024 7,245 4 View

My conditional effects are significant but my moderation analysis is not, how come?

So I am doing my thesis on the relation between cyberbullying perpetration and life satisfaction and if physical activity is a moderator. But in my moderation analysis the interaction is...

17 June 2024 6,400 2 View

2 Research Gate profiles?

Dear ResearchGate, I seem to have 2 independent profiles on your site which I do not know how could happen - can you, please, merge those into one? Both are on my name, but affiliations, however,...

04 June 2024 5,682 0 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

21 May 2024 4,964 4 View

Induction check BL21 DE3?

Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...

04 May 2024 3,549 2 View

The right to pre-trial release in the context of the constitution?

criminal procedure

29 April 2024 1,705 2 View

Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

28 April 2024 2,113 3 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Semaphorin3A treatment: How to use semaphorine to repulse axons and improve dendritic harborization in my culture chamber?

Good morning, I need to isolate dendrites from axons in my microchamber, I have already tried a protocol of Netrin and it failed. I would like to put Sema3a in my dendritic compartment in order...

26 March 2024 2,632 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Antonio Ahn

Around 24 hours? :D

Melanie LeVonne Grant

I haven't done this assay but suggest you do a concentration titration &/or time point comparison so you can pick the best option. Particularly if you're doing different cell lines, they may respond differently.

Anna L Leichter

Thanks guys will see how it goes!