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Questions related from Melanie LeVonne Grant
In my ELISPOT of tumor antigen-specific T cells we can see a very nice reduction in IFN-y (about half) in the presence of anti-Class I , suggesting a CD8-mediated response to peptide(s) in these...
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Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...
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Has anyone frozen tumor draining lymph nodes then thawed them to obtain T cells?Do you rest the T cells overnight or sort & use immediately? I have 1 colleague who rests human T cells...
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Has anybody measured the HOCl concentration in a NaClO solution? Do you have a good method?
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