Because we put femur bone for more than two months in EDTA and it still not calcified yet!!!
Hi, for a mice, it can take up to 4 weeks. A rabbit is much more bigger !
I advise you, if possible, to cut bone extremities, or cut it into 2 parts, to allow EDTA to get in. (you could also try the "osteosoft" product)
take a look on this group, which had work a while ago on rabbits: 10.1016/j.matbio.2007.07.006
https://www.leicabiosystems.com/knowledge-pathway/an-introduction-to-decalcification/
there are more type for mineral acid decalcifiers
with my regard
It also depends if you are using 10% or 14%. Best will be Follow Helene Follet's advise also to cut the sample in two so that EDTA can get in., and after some weeks keep on checking if it has become soft enough and can be cut with a scalpel
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