after 7 days 80% of our animals show tumor growth. I wonder for that 20% what should we do? does it need more time? Or second injection? or we should put them out of the experiment?
I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...
28 July 2020 6,711 1 View
High oil prices, environmental crises, and increasing energy demand have been the driving forces for rapid growth of renewable energy worldwide. All these forces have been weakened by COVID-19....
28 March 2020 3,752 20 View
Our power supply for western blotting transfer process needs three parameters of watt, voltage and current. In LC3 western blotting in different methods just the voltage or just the current are...
04 September 2019 8,137 1 View
Hi, I have done a seed to voxel analysis recently using conn toolbox. I have a basic question about how to report the result. My seed was a hippocampus structural mask and I compared its...
03 September 2019 5,822 1 View
-
10 March 2014 3,314 8 View
More or less similar policies are followed for renewable energy all over the world. Nevertheless, local conditions affect the formulation of an effective policy. In this context, what should be...
01 January 1970 8,580 14 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View