after 7 days 80% of our animals show tumor growth. I wonder for that 20% what should we do? does it need more time? Or second injection? or we should put them out of the experiment?
we recorded 6 log cfu/g total bacteria count in cheese. is it normal? A Fisherbrand Simple Phase Contrast Kit was used to evaluate the samples' total microbial counts.
02 April 2024 1,220 7 View
I want to calculate combination index (CI) for if two chemicals have synergistic effects or not.
23 September 2022 9,915 0 View
I want to do Live/dead cell viability assay on my collagen gels using calcein am and ethd-1 My questions are: a) What kind of plate for confocal microscopy should I use? I used thin glass bottom...
07 March 2022 5,196 4 View
I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...
28 July 2020 6,899 1 View
High oil prices, environmental crises, and increasing energy demand have been the driving forces for rapid growth of renewable energy worldwide. All these forces have been weakened by COVID-19....
28 March 2020 3,949 20 View
I am an urban design specialist with an emerging interest in "Smart Cities" concept and use of ICTs in the related fields (e.g. public spaces, urban planning, urban management, ...). I wanted to...
09 March 2020 5,754 8 View
It seems that the energy market is plunging due to the worldwide spread of Coronavirus. It is said that the virus threat would be over by the weather getting warm. Could it be a boost in the...
26 February 2020 780 20 View
Our power supply for western blotting transfer process needs three parameters of watt, voltage and current. In LC3 western blotting in different methods just the voltage or just the current are...
04 September 2019 8,290 1 View
Hi, I have done a seed to voxel analysis recently using conn toolbox. I have a basic question about how to report the result. My seed was a hippocampus structural mask and I compared its...
03 September 2019 5,961 1 View
Hi, I'm going run a simultaneous fMRI , eye tracking experiment. I expect having head some movement artefacts because of the eye tracking calibration. I think in multiband imaging we have two...
08 November 2018 9,768 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,827 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View