I read about DNA repair after exposure to UV light, photoreactivity is one of the mechanisms of repair. This mechanism is performed in the presence of light. I am wondering, if somebody exposure the spores suspension or vegetative growth (fungi grow on medium) to UV light how long need to left the plate or spores suspension in room to perform DNA repair?
Regards
Dear Ayat, that depends greatly on the fungus. UV repair could be super fast (minuets) in yeast but most of it is not contributed to photoreactivation. NER is a major factor here so if you want to isolate photorecativation you need to work in NER - background. Some fungi (can't remember - see work by Raymond St. Leger). Rely more on photoreactivation though. Look inside this paper for some answers J Bacteriol. 1972 Mar;109(3):1307-9.
You should also consider the irradiance and spectrum of the light used for reactivation. In plants, blue and UV-A radiation are most effective, but I am not familiar with details of the process in fungi. For UV exposure do take into account the UV-transmission properties of the vessels used for culturing the fungi.
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