Lauren Stranahan

6 Questions 4 Answers 0 Followers

Questions related from Lauren Stranahan

Recombinant protein production and alternative start codons?

I am using the pET-SUMO cloning system from ThermoFisher to recombinantly produce proteins in E. coli BL21(DE3) cells and have found success with several proteins expressed by the gram-negative...

20 July 2021 3,392 2 View

How can I reduce cleavage of my protein during expression in E. coli?

I am using the pET SUMO cloning system from Invitrogen to purify proteins in E. coli. BL21(DE3) cells are used for expression of the protein. Following induction with IPTG, I find that the E. coli...

29 October 2020 8,473 3 View

Double protein production with pET-SUMO cloning system?

Hi! I have been using the Champion pET-SUMO cloning system from Invitrogen to purify proteins in E. coli. I have noticed now with all of my proteins that during induction, there is overexpression...

15 October 2020 846 1 View

Purifying recombinant proteins in E. coli: signal peptide or no?

Hi! I am using the pET-SUMO system to purify recombinant proteins from Brucella spp. in BL21 E. coli cells and have had success with this method in purifying cytoplasmic proteins. My plans are to...

15 November 2019 9,162 8 View

Make your own SUMO protease?

Hi! I am cloning and purifying proteins using the pET SUMO Expression system from ThermoFischer. I have found that the supplied SUMO protease is not especially efficient and have read that...

29 July 2019 4,855 5 View

How can I avoid fibroblast contamination in BMMC culture?

I am trying to isolate and culture monocytes from canine bone marrow with the goal to differentiate them into macrophages. I am flushing the shaft of femurs from recently euthanized dogs (I know...

15 February 2018 8,563 2 View