Lauren Stranahan

3 Questions 3 Answers 0 Followers

Questions related from Lauren Stranahan

How can I reduce cleavage of my protein during expression in E. coli?

I am using the pET SUMO cloning system from Invitrogen to purify proteins in E. coli. BL21(DE3) cells are used for expression of the protein. Following induction with IPTG, I find that the E. coli...

29 October 2020 8,433 3 View

Double protein production with pET-SUMO cloning system?

Hi! I have been using the Champion pET-SUMO cloning system from Invitrogen to purify proteins in E. coli. I have noticed now with all of my proteins that during induction, there is overexpression...

15 October 2020 807 1 View

How can I avoid fibroblast contamination in BMMC culture?

I am trying to isolate and culture monocytes from canine bone marrow with the goal to differentiate them into macrophages. I am flushing the shaft of femurs from recently euthanized dogs (I know...

15 February 2018 8,519 2 View