Hi,
I work with an inflammatory model of Caco-2 cells. I seed the cells on transwell filter.
I establish the inflamation by adding the 10ng/ml of IL-6 in basolateral medium. I dont know this dose is enough any sugession?
After 6 hours exposure to cytokine I treat the apical part with my samples for 4 hours. I want to decided which way is best to check the anti inflamatory responses to my sample.
Which one of the following sugesstion is the best based on protein expression and inflamatory stimulation?
1. 10 hours IL-6 stimulation and in parallel 4 hour treatment with samples and continuing IL-6 stimulation for 14 hours more (total 24 hour) and ending the expriment and collect the sample?
2. 10 hours IL-6 stimulation and in parallel 4 hour treatment with samples and end the expriment (total 10 hours) and collect the sample?
Any better sugesstion and why?
Hi,
It was based on a response curve for a active phase protein.
Digested samles.
looking for inflamatory profile.
Thank you Frida. Do you have any result checking proinflammtory cytokines.
Please let me know you you know a published paper.
AgainThank you so much.
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