Shokoufeh Karimi

8 Questions 58 Answers 0 Followers

Questions related from Shokoufeh Karimi

Every cytokine has its own signaling pathway and don't effect other cytokines expression?

Is it correct to say that IL-6 has a seperate signaling pathway and don't effect expression of TNF -a in cell culture model ? Any answer appriciated.

07 March 2019 9,223 38 View

Can IL6 upregulate TNF-a and IL-8 in in vitro cell culture model?

Hi, I am working with an inflammatory cell culture model which the stimulater is IL6 10 ng /ml . But after checking the pro-inflammatory cytokines like TNF-a ,IL8, INF-a , non of them has been...

01 March 2019 4,290 4 View

A co-cultured stimulated IL-6 (An inflammatory model) does not show the upregulation of proinflammatory cytokines. Is the model working fine?

I am working on a in vitro inflammatory model(a co-cultured model) which stimulated with IL-6. After stimulation ,the control and control+IL-6 show no significatnt differences in expersion of...

07 February 2019 5,279 4 View

What is the best way to calculate the ELISA result?

I have used excel and Prism to analyse the results. I have some sample values which are below blank (OD). If I substract the OD value of these samples from blank I do get VERY LOW or negative...

03 February 2019 7,177 6 View

What is the best assay to confirm the result from ELISA?

Hi, I am checking the antiinflammatory effect of few substances on a co-culture model. I have screened the pro-inflammatory cytokines with ELISA kits. Do I need to confirm the result with...

23 January 2019 4,904 7 View

Can ELISA on cell lysate give more detectable results for cytokines compare to basolateral medium?

Can ELISA on cell lysate give better and detectable results for cytokines compare to basolateral medium?

09 January 2019 6,750 3 View

Does phenol red interfer with ELISA?

Hi, I am going to run an ELISA- a colorimetric based method. I have phenol red in my samples. I read both negative and nutral impact of phenol red on ELISA. I want to know if really phenol red...

07 December 2018 3,252 3 View

How long is a proper time for protein amount measurment and establishment of an inflammation using IL-6 in in vitro cell culture model?

Hi, I work with an inflammatory model of Caco-2 cells. I seed the cells on transwell filter. I establish the inflamation by adding the 10ng/ml of IL-6 in basolateral medium. I dont know this dose...

27 November 2018 8,702 4 View