Why I got smear in my PCR product using kod plus?

10 November 2016 6,515 5 View

How to separate 404 bp with 344 bp for dna product PCR?

08 September 2016 1,243 3 View

What the reason rasio vector : dna is 1: 90 by using vector p.BluecriptSK-II-ecoRV?

06 July 2016 950 7 View

Can anyone help with running gel for purification by gel extraction?

Dear All I have done gel extraction my PCR product (2300bp), but the final I lose all my DNA. Here my following step. Ethanol precipitation (using glycogen, Na-OCH, ethanol) My PCR product 50...

06 July 2016 4,244 4 View

Is no problem if I do Ethanol precipitation with composition glicogen, Na-asetat, before and after phosphorylation PCR product by T4 PNK ?

Dear all, Is there anyone have experience do ethanol precipitation PCR product prior to phosphorylation by T4 polynucleotide kinase? According literature ethanol precipitation by ammonium asetat...

06 July 2016 3,195 3 View

How to design phosphorylation PCR product ?

Dear ALL I want to phosphorylate my PCR product, please give me sugestion how to calculate p mol PCR product.., is there any protocol without converse.., is there any link or literature I can use...

06 July 2016 5,668 1 View

Is it possible to Clone ArfGAP protein with SH3 domains, ankyrin repearts & pleckstrin homology domains (ASAP1) in neuron brain mouse tissue?

Dear All I have plan to clone ASAP1 from neuron brain mouse tissue. Is there anyone have experience cloning ASAP1 with cDNA from neuron brain mouse? I already design primer and check by primer...

05 June 2016 216 0 View

The meaning any, self, pair any and pair End by using primer3plus ??

04 May 2016 9,290 3 View

How to solve Unexpected band after amplification PCR ?

I have done amplification by PCR for my gene, but unexpected I got several band higher expression than my band, I have try changed my condition annealing time (Tm) but the result still same,...

04 May 2016 7,280 20 View

How to check cDNA mouse embryonic fibroblast gene expression ?

Dear All Is there any software to know gene expression high or low in mouse embryonic fibroblast? Best Regards

03 April 2016 1,123 12 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View