Capsicum annuum seeds were placed in MS media 5.8 pH on February 6th, no significant changes can be seen so far. The seeds were planted via in vitro and the containers placed under white germination lights at room temperature (around 10 to 30°C).
1. How old are those seeds? Long-term stored seeds can lost their germination ability.
2. See this link (World of chillies): https://www.worldofchillies.com/Chilli-plant-varieties/Chilli-plant-varieties-Capsicum/Chilli-plants-Capsicum-Annuum.html
It says:
"For best results, it is important to make sure that the temperature of the newly planted seeds does not drop lower than 65 F (or 18C)."
In what kind of containers are you placing the seeds. It took me lesser time when I did in tubes where cotton plug was used. The seeds took a longer time to germinate when I kept them in bottles with plastic caps. Kindly make sure the plates are not too warm. All the best.
The seeds were about a month old and extracted directly from dried chilies. I used glass containers to place the seeds Gayathri Subramanian. Since your suggestion Yuan-Yeu Yau I changed the containers with the seeds to a controled temperature room and I have been checking on them every day. Sadly, the seeds have not germinated yet. I'm hoping to see some changes in a couple of days. Thanks a lot for your advice, I'll keep you informed about any changes.
Hello Luis
When seeds do not germinate on a medium like MS, three explanations could arise: they are dead or they are dormant or the temperature used is inadequate (what is often linked with the state of dormancy, because the more seeds are dormant, the narrow is the permissive temperature window for germination to occur.) As your seeds are freshly produced, they are probably dormant and cannot germinate at the temperature used (generally Chilli seeds don’t like T° below 12°C). Seed dormancy can be broken in different ways: after-ripening, cold stratification, light, hormonal treatments like gibberellins or ethylene or fluridone (in this last case plantlets will be chlorotic but can overcome to green after transfer).
See you.
@ Luis Fuente Cevallos,
1. Make sure that when you sterilize the seeds with EtOH or/and bleach, you don't sterilize them too harsh, which can kill the seeds. Follow a good protocol from an good journal.
2. You can grow some seeds in soil to see whether they germinate or not.
Here, also an informative report: Pepper Seeds Won't Germinate? Some Tips.
[ http://farmerfredrant.blogspot.com/2012/03/pepper-seeds-wont-germinate-some-tips.html?m=1 ]
They mentioned that pepper seeds are not as easy as tomato seeds to germinate. It can take 2-3 weeks under 80F (~27C) or 85F to germinate in soil. Read the whole article and the comments from other people below the article. Some people even used 'heating mat' to help germination. It is interesting. I think temperature seems to play an important role in pepper germination. So, you can wait a little longer to see whether your seeds germinate. Update us.
After 17 days of being placed in MS media and about a week under controlled temperature, the seeds finally sprouted. Thanks a lot for your help and advice Yuan-Yeu Yau , Jullien Marc . Here’s a picture of the sprouted seeds:
Nice, thank you for sharing with us!!
By the way, what controlled temperature did you use?
As your seeds germinated slowly we can say they were in a relatively dormant. Stored dry some times at room temperature (afterripened) they will probqably germinate more quickly...
See you.
Yuan-Yeu Yau I used 30°C in a controlled temperature room. Jullien Marc Thanks for the advice!
It will take 10 to 12 days when the cultures are incubated in 23 to 25 degree celsius using test tube cotton plug encloser and placed them as 45 degree angel.
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