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I ve started with culturing HCN-2 cells. I start this by coating 10 cm dish with Poly-L-ornithine for 24 hours. After that, I removed the excess liquid and washed one time with PBS. After that, I...
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At first, we performed transfections. To analyze that transfection, we performed RNA isolation, cDNA synthesis and qPCR. Using the qPCR, we analyze multiple samples, whereof each sample we...
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I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...
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