Martijn Koetsier

4 Questions 3 Answers 0 Followers

Questions related from Martijn Koetsier

Can HEK293 cells survive a 30-minutes transport and 2h FACS experiment in RT PBS?

I have to do a FACS experiment with my HEK293 cells, but we have to do this experiment in an other building. Can I transport them 30 minutes in RT PBS (on ice better?) and doing a FACS experiment...

31 August 2020 5,640 3 View

How long does it take for HCN-2 attach to the plate?

I ve started with culturing HCN-2 cells. I start this by coating 10 cm dish with Poly-L-ornithine for 24 hours. After that, I removed the excess liquid and washed one time with PBS. After that, I...

30 July 2020 286 0 View

Why differs CT values of one sample between its triplos (Taqman Assays and qPCR)?

At first, we performed transfections. To analyze that transfection, we performed RNA isolation, cDNA synthesis and qPCR. Using the qPCR, we analyze multiple samples, whereof each sample we...

20 March 2020 2,596 4 View

What is the optimal method to the cryopreservation of SH-SY5Y cells?

I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...

15 November 2019 3,723 3 View