I have amplified the fungal ITS gene and because of the current emergency situation, I'm unable to send the PCR product for sequencing. So, I stored them at below -10°C. I want to know How long can I store them.
Using adequate storage condition at -20°C (better -80°C), it should be stable and of adequate quality for at least 6 month. At 4°C at least 1 (or more) week(s) storage should also be ok.
Using adequate storage condition at -20°C (better -80°C), it should be stable and of adequate quality for at least 6 month. At 4°C at least 1 (or more) week(s) storage should also be ok.
I agree with Andreas Eisenreich that the pcr products will be stable at both -20 and -80 for a very long time. I would say for at least a year and probably much longer but very certainly good for 6 months. They are best stored in the dark and should not be thawed many times so if your freezer is self de-icing put them in the middle of the freezer not close to the cooling coils
Thank you so much for the suggestions Andreas Eisenreich and Paul Rutland
I agree with the previous responses. You can store PCR products at -4 degree C for 2 weeks, -20 degree C for up to 6 months and -80 degree C for long time storage until you perform sequencing PCR.
You can store at -80 degree C as suggested by Charles D Anderson and others since you do not know when you would send it for sequencing.
In our lab we have stored our samples in -4 degree centigrade temperature for 2-3 weeks. In -20 degree centigrade temperature the samples can be stored for long time. I get results from the samples which are stored in this temperature for more than 1 year.But you should ensure that there is no electricity or voltage issue.
Obtained good MiSeq results for the DNA samples stored at -20 for 6 months.
-20 degrees is fine. I agree with the other responses.Try to avoid too many freeze -thaws
You can store PCR product till one week in room temperatur, for one or two months kept in 4 and for longer till one year store at - 20 degree cetigrade.
I have sequenced my PCR product after a month or few days above. It worked pretty well. And it was stored at -4C. But I never let it freeze/thaw for several times.
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