I have some liquid cultures of individual colonies that have been grown in LB plus kanamycin [50ug/ml]. I want to extract plasmid DNA from them all in one big batch. To do this, I was going to grow all colonies overnight, then remove them from the incubator and leave them on my bench for a few days before isolating the plasmid DNA. Will this be ok? Do I need to store them differently to prevent problems? I don't want to go to all the effort of producing them if I cannot use them!
Thank you!
Hi, Emma. I'd leave them at 4 degrees instead of on your bench, but other than that your plan sounds fine. (Actually, a few days on the bench would probably be OK too).
Emma,
I recently left them few days as it in the freezer, I've got a good yield from my pDNA extraction.
Thanks for all your answers. I asked as I have 170 cultures and only one pair of hands! I have isolated all the plasmid DNA now in batches, it did take a few days but I didn't have much choice on a practical scale - I will take this into consideration in the future planning and see how these isolations work as well. Thanks again all!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- DNA