I know that S.cerevisiae suspension can be kept at 4℃ for a few weeks in liquid media such as
YPD or SD media.
However, I'm unsure whether I can store yeast cells in PBS for a few days to weeks without
losing viability. It seems like animal cells cannot be stored for more than a few hours.
On the other hand, bacteria can survive in PBS for weeks to months. So, I'm uncertain.
I'm planning to store my budding yeast in PBS at 4℃ over the weekend and continue my
experiment with WGA staining for counting bud scars.
I propose freezing them in their media, supplemented with 10% DMSO. the freezing temperature -70 degree centigrade is favorable.
I think that other than the optimal cultivation temperature could change its original glycoprotein phenotypes to some extent, so I would like to recommend please keep your samples at 4C after staining and fixation.
Yeast can be stored in the freezer to extend its life for many months without affecting its working efficiency; Freezing the yeast cells instead of damaging them
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining