I will be receiving a lot of whole blood samples and would like to know the best way to store them for DNA extraction. Also, how long I can keep the samples in storage?
Merin, you know how long blood samples stored in EDTA can be kept at ambient temperature?
Blood can be collected into tripotassium EDTA; citrate, theophylline, adenosine, and dipyridamole (CTAD); or a combination of both (E/C) and stored at ambient temperature or at 4 °C (E/C only) and can be analyzed between 0 and 180 min later on the ADVIA 120. It can be stored for 12, 24 or 36 h prior to processing at 4°C and it can be frozen at −80°C for 20 days and then thawed under controlled conditions. Stability of the samples can different based on variety of assays used.
Blood can be collected into tripotassium EDTA; citrate, theophylline, adenosine, and dipyridamole (CTAD); or a combination of both (E/C) and stored at ambient temperature or at 4 °C (E/C only) and can be analyzed between 0 and 180 min later on the ADVIA 120. It can be stored for 12, 24 or 36 h prior to processing at 4°C and it can be frozen at −80°C for 20 days and then thawed under controlled conditions.
Blood can be collected into tripotassium EDTA; citrate, theophylline, adenosine, and dipyridamole (CTAD); or a combination of both (E/C) and stored at ambient temperature or at 4 °C (E/C only) and can be analyzed between 0 and 180 min later on the ADVIA 120. It can be stored for 12, 24 or 36 h prior to processing at 4°C and it can be frozen at −80°C for 20 days and then thawed under controlled conditions.
Blood can be collected into tripotassium EDTA; citrate, theophylline, adenosine, and dipyridamole (CTAD); or a combination of both (E/C) and stored at ambient temperature or at 4 °C (E/C only) and can be analyzed between 0 and 180 min later on the ADVIA 120. It can be stored for 12, 24 or 36 h
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