How to resuspend DNA that is currently in elution buffer into MilliQ water?

11 December 2014 1,380 6 View

Does anyone have any advice about baffling low concentrations of DNA on Qubit ?

I extracted whole blood samples using the Qiagen DNeasy blood and tissue kit. When quantifying the DNA on the Qubit, I get baffling low DNA concentrations. Is there something that I might be doing...

10 November 2014 6,068 5 View

How long can whole blood (EDTA tubes) be stored and at what conditions?

I will be receiving a lot of whole blood samples and would like to know the best way to store them for DNA extraction. Also, how long I can keep the samples in storage?

01 February 2014 7,853 7 View

Best resources for cell culturing and tissue models?

Hi all, I would like to know what you consider as the best resources for cell culturing and tissue modeling for a newbie? Thanks so much

01 January 1970 6,620 3 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

EDGAR - How to add Whole genome sequence of the strain (novel) to EDGAR comparative genomic tool for comparison?

Hi all, I am interested to do a comparative genome analysis using EDGAR comparitive genomic tool. I have a novel bacteria whose genome sequence was obtained by us and now I was to do compare this...

10 November 2020 3,489 3 View

Which methods (or modern programms) are used to assess the biodiversity of highest vascular plants?

27 October 2020 240 3 View

R package for GLMM with phylogeny (but not for community composition)?

Hello, I have a dataset derived from a multi-species experiment that I carried out. I have 30 different plant species from several families and 3 geographical groups. I applied 6 different...

26 October 2020 874 3 View

How can I perform pairwise protein alignments using BLAST, but without search?

I have a set of about 1000 homologous protein sequences with high diversity across various genera and families. What I need is to perform BLAST alignment of each possible sequence pair and obtain...

25 October 2020 1,199 5 View

What are the genetic/physiological differences between deep water rice cultivars of North-Eastern India from that of South East Asian Countries?

Deepwater rice cultivars found in various regions around the world have their own differences. Please mention how the deepwater rice cultivars of North-Eastern India varies from that of Southeast...

23 October 2020 1,333 3 View

Has anybody used XLSTAT software for phylogenetics/Phylogeography?

Need a detailed tutorial on XLSTAT ( Input type format, output file, drawing conclusions etc) Unable to find online.

07 October 2020 6,764 4 View