Dear All,
My recipe of 5X SDS-PAGE sample buffer without beta mercaptoethanol is 156.25 mM Trish-HCl, pH 6.8, 62.5% Glycerol, 5% SDS, 0.025% Bromophenol Blue.
How long should the sample buffer be stored in room temperature? SDS and Glycerol should be fine in room temperature. But I am not sure about Tris-HCl inside the buffer will it be ok in room temperature for 2-3 months.
Thank you
Cheerios,
Nothing of the components should be affected at room temperature, but we usually keep the loading buffer in the fridge or at freezer.
Two years.
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006028.pdf
Non of the components should affected. I usually store it for a year at room temperature an the efficacy doesn´t change. You must avoid store it at the fridge because the sds precipitate under 10ºC
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