Has anyone sampled for RNA viruses in sub-tropical conditions using FTA cards?
The manufacturer recommends RNA analysis upon return to the laboratory.
An alternative is the Fortiusbio card, which extends the preservation of RNA to a few days.
http://www.fortiusbio.com/RNA_Sampling_Card.html
Please recommend any free NGS data analysis software that runs on Windows. I have downloaded and used CLC Genomics Workbench, but only for two weeks. Any other software that I can use for longer...
11 December 2016 2,080 22 View
I ran a gradient PCR and got multiple bands on all the temperature regimes that I had programmed. Why was this the case? How can I correct this so that I get the optimum temperature?
02 March 2015 9,239 6 View
I have been using gene specific primers for PCR. The primer synthesis report gave a lower Tm (by 7 degrees Celsius) than has been used elsewhere for the same primers. I have tried to optimize, but...
01 February 2015 6,055 19 View
RNA Analysis
07 August 2014 8,353 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View