Charles Karavina

5 Questions 10 Answers 0 Followers

Questions related from Charles Karavina

Any free NGS data analysis software that runs on Windows?

Please recommend any free NGS data analysis software that runs on Windows. I have downloaded and used CLC Genomics Workbench, but only for two weeks. Any other software that I can use for longer...

12 December 2016 2,054 22 View

What causes multiple bands in gradient PCR?

I ran a gradient PCR and got multiple bands on all the temperature regimes that I had programmed. Why was this the case? How can I correct this so that I get the optimum temperature?

03 March 2015 9,209 6 View

How do I determine primer annealing temperature?

I have been using gene specific primers for PCR. The primer synthesis report gave a lower Tm (by 7 degrees Celsius) than has been used elsewhere for the same primers. I have tried to optimize, but...

02 February 2015 6,030 19 View

Does viral RNA maintain infectivity when preserved in a nucleic acid preservation solution like RNAlater?

RNA Analysis

08 August 2014 8,310 4 View

How long can RNA be stored on FTA (Whatman) cards?

Has anyone sampled for RNA viruses in sub-tropical conditions using FTA cards?

11 November 2013 6,555 1 View